flag agarose beads Search Results


anti flag magnetic beads  (MedChemExpress)
95
MedChemExpress anti flag magnetic beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Anti Flag Magnetic Beads, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/Anti-Flag+Magnetic+Agarose+Beads/pmc11005161-161-21-24
Average 95 stars, based on 1 article reviews
anti flag magnetic beads - by Bioz Stars, 2026-09
95/100 stars
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anti ddk agarose beads  (OriGene)
92
OriGene anti ddk agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Anti Ddk Agarose Beads, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/Clone+OTI4C5%2C+Anti-DDK+(FLAG)+Monoclonal+Antibody%2C+Agarose+Beads/pm28289210-303-0-3
Average 92 stars, based on 1 article reviews
anti ddk agarose beads - by Bioz Stars, 2026-09
92/100 stars
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anti-flag m2conjugated agarose beads  (GenScript corporation)
90
GenScript corporation anti-flag m2conjugated agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Anti Flag M2conjugated Agarose Beads, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/anti+flag+m2conjugated+agarose+beads/pm38599381-408-13-17
Average 90 stars, based on 1 article reviews
anti-flag m2conjugated agarose beads - by Bioz Stars, 2026-09
90/100 stars
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anti-flag agarose beads  (AlpaLife Inc)
90
AlpaLife Inc anti-flag agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Anti Flag Agarose Beads, supplied by AlpaLife Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/anti+flag+agarose+beads/pm37956198-341-18-21
Average 90 stars, based on 1 article reviews
anti-flag agarose beads - by Bioz Stars, 2026-09
90/100 stars
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flag agarose beads genscript g1 a-dykddddk  (GenScript corporation)
90
GenScript corporation flag agarose beads genscript g1 a-dykddddk
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Flag Agarose Beads Genscript G1 A Dykddddk, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/flag+agarose+beads+genscript+g1+a+dykddddk/pmc06557285-102-5-8
Average 90 stars, based on 1 article reviews
flag agarose beads genscript g1 a-dykddddk - by Bioz Stars, 2026-09
90/100 stars
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flag–agarose beads  (GenScript corporation)
90
GenScript corporation flag–agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Flag–Agarose Beads, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/flag+agarose+beads/pm30867594-367-8-7
Average 90 stars, based on 1 article reviews
flag–agarose beads - by Bioz Stars, 2026-09
90/100 stars
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flag-agarose beads  (Promega)
90
Promega flag-agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Flag Agarose Beads, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/flag+agarose+beads/pm16166097-71-8-11
Average 90 stars, based on 1 article reviews
flag-agarose beads - by Bioz Stars, 2026-09
90/100 stars
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anti-flag agarose beads  (ApexBio)
90
ApexBio anti-flag agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Anti Flag Agarose Beads, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/anti+flag+agarose+beads/pm27273514-665-5-17
Average 90 stars, based on 1 article reviews
anti-flag agarose beads - by Bioz Stars, 2026-09
90/100 stars
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anti-flag antibody coupled agarose beads  (AlpalifeBio Inc)
90
AlpalifeBio Inc anti-flag antibody coupled agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Anti Flag Antibody Coupled Agarose Beads, supplied by AlpalifeBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/anti+flag+antibody+coupled+agarose+beads/pm40345159-368-0-8
Average 90 stars, based on 1 article reviews
anti-flag antibody coupled agarose beads - by Bioz Stars, 2026-09
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flag antibody-conjugated agarose beads  (AlpaLife Inc)
90
AlpaLife Inc flag antibody-conjugated agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Flag Antibody Conjugated Agarose Beads, supplied by AlpaLife Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/flag+antibody+conjugated+agarose+beads/pm39537119-106-16-20
Average 90 stars, based on 1 article reviews
flag antibody-conjugated agarose beads - by Bioz Stars, 2026-09
90/100 stars
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flag agarose beads  (FUJIFILM)
90
FUJIFILM flag agarose beads
SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with <t>anti-FLAG-C</t> or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.
Flag Agarose Beads, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flag+agarose+beads/flag+agarose+beads/pm24103748-51-0-11
Average 90 stars, based on 1 article reviews
flag agarose beads - by Bioz Stars, 2026-09
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Image Search Results


SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with anti-FLAG-C or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.

Journal: Veterinary Research

Article Title: Occludin and collagen IV degradation mediated by the T9SS effector SspA contributes to blood–brain barrier damage in ducks during Riemerella anatipestifer infection

doi: 10.1186/s13567-024-01304-y

Figure Lengend Snippet: SspA binds to occludin. A SspA expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 2: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). B SspA has hydrolytic activity towards gelatine (Lane 1: 2 µg of recombinant rSspA protein; Lane 2: lysate of 293 T cells; Lane 3: lysate of 293 T cells transfected with the plasmid pcMV- flag ; Lane 4: lysate of 293 T cells transfected with the plasmid pcMV- sspA - flag ). C Occludin expression in 293 T cells (Lane 1: lysate of 293 T cells transfected with the plasmid pcDNA -myc ; Lane 2: lysate of 293 T cells transfected with the plasmid pcDNA -occludin-myc ). D The subcellular distribution of SspA (red) and occludin (green) was examined by immunostaining with anti-FLAG-C or anti-MYC-C antibodies. Nuclei were stained with DAPI (blue). Merged images showing SspA/occludin/DAPI fluorescence. E Immunoprecipitation of SspA and occludin. 293 T cells were co-transfected with pcMV -sspA-flag and pcDNA -occludin-myc. The cell lysates were incubated with anti-Flag magnetic beads and subjected to immunoprecipitation using an anti-MYC-C tag polyclonal antibody. The precipitated proteins and whole-cell lysates were analysed by Western blotting.

Article Snippet: The cell lysates were centrifuged at 12 000 rpm for 20 min. Next, the supernatants were incubated with 50 μL of Anti-Flag Magnetic Beads (MedChemExpress, USA) at 4 °C overnight.

Techniques: Expressing, Transfection, Plasmid Preparation, Activity Assay, Recombinant, Immunostaining, Staining, Fluorescence, Immunoprecipitation, Incubation, Magnetic Beads, Western Blot